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Commentary

The History of MS-Patch-Clamp Technique Development: Integration of Mass Spectrometry and Membrane Electrophysiology

Gradov Oleg V

CHEMBIO Department, N.N. Semenov Federal Research Centre For Chemical Physics Of The Russian Academy Of Sciences (FRC CP RAS), Moscow, Kosygina 4, 119991, Russia


Recieved on: 2024-11-01, Accepted on: 2024-12-04, Published on: 2024-12-11

Abstract

The history of MS-patch-clamp technique development as integration of single-cell mass spectrometric and membrane electrophysiological instrumental approaches (from the integration of patch-clamp electrophysiology and capillary electrophoresis-mass spectrometry and primary MS-patch-clamp proposals to the novel single-lysosome mass spectrometry techniques (SLMS) and rapidly progressing area of combined single-cell-level mass-spectrometry and capillary electrophoresis for the analysis of activity of excitable living tissues) has been considered. The author expresses a desire for international collaborations to advance the development of MS patch clamp methods and related techniques in the future. The material of this short communication was initially planned as a presentation at the conference on mass spectrometry in 2024, but the inability to obtain a visa from Russia and financial difficulties associated with the international sanctions made this presentation impossible.

Keywords

MS-patch-clamp techniques; Ion channels; Patch-clamp electrophysiology; Capillary electrophoresis-mass spectrometry; Single-lysosome mass spectrometry techniques; Single-cell-level mass spectrometry; Single-cell proteomics; Single-cell metabolomics